Biolayer Interferometry (BLI) is an established method that is traditionally used to study biomolecular interactions. This label-free technique measures the binding of biomolecules to the tip of a biosensor in real-time to determine kinetic rates of interaction pairs or to quantify biomolecular samples. The newest generation of AAV biosensors from Gator Bio opens up this application to the field of gene therapy and enables accurate and reliable quantitation of AAV total capsid titer. With the AAVX and AAV9 biosensors and the new high-sensitivity kit, Gator Bio covers AAV serotypes 1 – 10 with a dynamic range from 5E+6 to 1E+12 vp/ml.
The system is robust enough to measure in crude extracts, cell medium or purified samples and can work with only 40 µl of sample. Biosensors can be automatically regenerated and reused to reduce costs per assay. The intuitive software with straight forward assay setup and analysis make this application easy to learn, even for unexperienced lab members. The mayor strengths of the Gator Bio devices are the high accuracy, short assay time and wide dynamic range.
High accuracy
Gator Bio directly detects binding of biomolecules to the sensor surface in real-time, without the need for additional labels, antibodies or other indirect detection methods. Together with the new generation of precoated AAV biosensors, the high sensitivity of BLI makes the accuracy of Gator Bio superior to ELISA.
Convince yourself of the high accuracy of the Gator Bio BLI systems and make a demo appointment with our product specialists!
Short assay time
Measure up to 8 samples in parallel within 2 minutes. With minimal preparation time, the Gator Bio system can quantify 96 samples in under 30 minutes. The straight forward data analysis in the integrated software enables you to get results immediately after the assay.
Convince yourself of the short assay times of the Gator Bio BLI systems and make a demo appointment with our product specialists!
Wide dynamic range
There is no need to dilute your samples, no matter if you are in early growth, purification or QC step. This reduces the hands-on time and eliminates errors from the sample dilution steps.
Convince yourself of the wide dynamic range of the Gator Bio BLI systems and make a demo appointment with our product specialists!
Additional features
- Robust: Gator Bio can quantify AAV samples in crude extracts, cell medium or after purification.
- Low maintenance: Probes and sample plates are disposable. There is no need for extensive cleaning protocols or instrument maintenance.
- Cost effective: Probes can be regenerated automatically to decrease the cost per assay.
- Little material needed: Quantitation can be performed with only 40 µl per sample.
- Low sample consumption: The Measurement is non-invasive. Precious sample material can be recovered after quantitation.
- User friendly: The intuitive software enables easy assay setup and analysis.
Gator Bio BLI systems for AAV applications
GatorPlus
Next gen label-free BLI instrument with high sensitivity
GatorPlus is a next generation Biolayer Interferometry (BLI) system. Among the applications are label-free quantitation and kinetic analysis of antibodies, AAV, proteins, peptides, nucleic acids, aptamers, liposomes, viruses, small molecules and epitope binning/mapping. The system uses the 384-well plate format and offers best-in-class baseline stability for higher sensitivity.
GatorPrime
Next Gen label-free Biolayer interferometry (BLI) instrument
GatorPrime is a next generation Biolayer Interferometry (BLI) system. Among the applications are label-free quantitation and kinetic analysis of antibodies, AAV, proteins, peptides, nucleic acids, aptamers, liposomes, viruses, small molecules and epitope binning/mapping. This system uses a variety of new biosensors to make assay setup and performance as fast and easy and possible.
