Discover the flexible technique that accurately determines AAV titer and Empty/Full Ratios in purified or crude samples.
Gator Bio directly detects binding of biomolecules to the sensor surface in real-time, without the need for additional labels, antibodies or other indirect detection methods. Together with the new generation of precoated AAV biosensors, the high sensitivity of BLI makes the accuracy of Gator Bio superior to ELISA.
Measure up to 8 samples in parallel within 2 minutes. With minimal preparation time, the Gator Bio system can quantify 96 samples in under 30 minutes. The straight forward data analysis in the integrated software enables you to get results immediately after the assay.
There is no need to dilute your samples, no matter if you are in early growth, purification or QC step. This reduces the hands-on time and eliminates errors from the sample dilution steps.
The approach involves three steps:
1. AAV capture on AAVX or AAV9 probes
2. Transfer and lysis of AAV particles to release the ssDNA
3. Quantitation of released ssDNA with ssDNA probes
The figure shows ssDNA binding signal for lysed AAV2 capsids at the same concentration. The ssDNA signals are proportional to the corresponding Full Ratio of the sample.