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PasLite Phosphatase Test

The PasLite test is an internationally accepted method used by dairies and food manufacturers to verify HTST pasteurization for many types of dairy products, including fluid white milk, flavored dairy, non-flavored dairy, and solid/semi-solid dairy (like cheese and ice cream). The PasLite test verifies the completeness of milk pasteurization by detecting alkaline phosphatase, a natural enzyme in milk that is destroyed by the heat and hold time of pasteurization. The test takes 3 minutes and multiple samples can be run simultaneously.

When a dairy sample is mixed with PasLite reagents and incubated, the resulting solution emits light in an amount directly proportional to the phosphatase enzyme present. The Charm novaLUM II-X system is used to measure the light emitted and converts light readings to enzyme units. Phosphatase readings greater than 350 mU/L indicate product pasteurization issues according to US and EU pasteurization requirements.  The PasLite test limit of detection for liquid dairy products is 20 milliunits per liter (mU/L) phosphatase (approximately 0.002% raw milk). This is well below the 350 mU/L level (0.1% raw milk) required by most public health agencies.

Technical data

 

 

Details Result
Time to result 3 Minutes
Limit of detection 20 mU/L
Detection sensitivity milliunits per litre
Pack sizes Available in 20 or 100 test strip packs
Recommended reader Charm Science novaLUM II-X Luminometer

 

 

 

Consumables

 

 

Charm Science novaLum 2X Luminometer  Charm Sciences Inc.
Digital Dry Block Incubator Charm Sciences Inc.

 

Technical Service


During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.

Detailed information about our support options can be found here.

Charm Sciences Inc.

Founded in 1978, the company has grown from just a few employees in a small Boston loft space to 3 facilities. Today, the three factories in Massachusetts produce all reagents, equipment, and even software and firmware, and is the global leader in manufacturing rapid diagnostic tests to detect residues, toxins, microbial, and sanitation risks in a wide range of industries, including food and beverage, hospitality, healthcare, pharmaceutical, and personal care. Charm develops fast and reliable test methods that are easy to perform, delivering unmatched quality with a commitment to customer satisfaction. Due to their vertical integration, Charm has the flexibility to offer customized solutions to solve customer problems quickly.

More about Charm Sciences: www.charm.com

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131 employees
10 locations in europe
31 years of experience