Label-free biomolecular interaction analysis is aimed at understanding the connections among biomolecules and the outcomes arising from these specific associations. This form of analysis plays a pivotal role for medical research and in the pharmaceutical industry. The interactions between biomolecules are of great importance to understand many biological processes. Although certain proteins operate independently, the majority of proteins are only active when they form complexes with other molecules. Biomolecule interactions are fundamental to all cellular processes such as signal transduction and metabolic pathways.
Gator Bio has introduced a new instrument platform for label-free biomolecular interaction analysis, bringing BLI to the next level. Discover how you can accelerate your biotherapeutics research and drug development with the Gator Bio BLI instruments.
BLI is an optical analytical approach based on the reflection of white light on the surface of a biosensor tip. A shift in the interference pattern occurs when the number of molecules bound to the biosensor tip is changed. The interactions are determined in real-time and analysis of binding specificity, association-dissociation ratios (kinetics) and concentration are measured with high accuracy and reliability. Only bound molecules to the biosensor affect the interference pattern, meaning that non-binding molecules have no effect on the pattern. Consequently, this allows the utilization of crude samples for measurement.
Figure 1 demonstrates how the signal intensity of the reflection beams is translated into a graph, where you can easily monitor the changes in the binding of molecules to the sensor surface.
Figure 1. Schematical overview of the different steps of BLI used for a kinetics experiment. Binding is measured real-time, without the need of a label. A. The baseline is set by the reflected light of the surface chemistry. B. The sensor is loaded with the binding ligand. C. The target molecule binds to its binding partner. D. Release of the target molecule.
Figure 2. Kinetic analysis of a purified antigen-antibody pair using Gator Bio Streptavidin biosensors.
This graph shows the different steps involved in a standard kinetics experiment (loading, association, dissociation).
BLI is a versatile technology that can be used for a range of applications and a wide variety of biomolecules. The most common target molecules researched using BLI are: antibodies, proteins, peptides, small molecules, viral vectors, DNA and lipid nanoparticles.
Measure the concentration of biomolecules such as proteins and antibodies in crude cell culture samples, production harvests, crude lysates or purified samples.
The high-throughput capability and short assay time of BLI makes it very useful for antibody production and development, for example with antibody quantification in supernatants or clone selection.
BLI is a very powerful and easy-to-use technique to measure binding kinetics such as association rate and dissociation rate between a biomolecule and its binding partner (on- and off-rates). In addition, affinity strength (dissociation constant) can be determined.
In antibody drug development, knowing the epitope of an antibody is very important to understand the therapeutic potential. Running epitope binning analysis early on in the drug development pipeline can increase efficiency and save time in the overall drug development process.
In addition, epitope binning can be used to find matching antibody pairs for diagnostic test development and is also used to understand vaccine functionality during vaccine research.