Determination of AAV total capsid titer and empty/full ratio using Gator Bio's biolayer interferometry (BLI) technology
Consistent AAV analytics during the manufacturing process is challenging due to a lack of standardized methods that can cover all process steps. Biolayer Interferometry (BLI) offers a flexible technique with various applications throughout the process including capsid titer, empty/full ratio determination and Tab and Nab assays. Gator Bio's AAV analytics solutions offer several advantages that make them suitable for integration into manufacturing processes from start to finish: crude sample compatibility, high sensitivity, accuracy, robustness, automation, and reduced hands-on time. With Gator Bio's AAV analytics solutions, you can expect reliable and accurate data to optimize your processes and produce high-quality products. By leveraging the flexibility and robustness of BLI, you can now streamline your AAV analytics and maintain consistency throughout the manufacturing process.
Gator Bio directly detects binding of biomolecules to the sensor surface in real-time, without the need for additional labels, antibodies or other indirect detection methods. Together with the new generation of precoated AAV biosensors, the high sensitivity of BLI makes the accuracy of Gator Bio superior to ELISA.
Measure up to 8 samples in parallel within 2 minutes. With minimal preparation time, the Gator Bio system can quantify 96 samples in under 30 minutes. The straight forward data analysis in the integrated software enables you to get results immediately after the assay.
There is no need to dilute your samples, no matter if you are in early growth, purification or QC step. This reduces the hands-on time and eliminates errors from the sample dilution steps.
The approach involves three steps:
1. AAV capture on AAVX or AAV9 probes
2. Transfer and lysis of AAV particles to release the ssDNA
3. Quantitation of released ssDNA with ssDNA probes
The figure shows ssDNA binding signal for lysed AAV2 capsids at the same concentration. The ssDNA signals are proportional to the corresponding Full Ratio of the sample.
Next Gen label-free Biolayer interferometry (BLI) instrument
The GatorPrime is our entry level instrument that brings next generation BLI technology into your lab. This versatile 8-channel instrument uses our variety of new high-performance biosensors and is ideal for groups that are working on a budget.
Next gen label-free BLI instrument with high sensitivity
The Gator Plus is our advanced 8-channel system that provides you with the additional option to use 384 well plates. Increase your run times and significantly reduce your sample volume while still getting the same high-quality data sets from our innovative biosensors.
The most powerful BLI instrument in the world
The Gator Pro is our 32-channel system that puts high-throughput to the next level. Equipped to handle 3 x 384-well plates and ready for automation. Finish your 32 x 32 Epitope binning experiments in just 8 hours without suffering a loss in data quality.