Determination of AAV total capsid titer and empty/full ratio using Gator Bio's biolayer interferometry (BLI) technology
Discover the flexible technique that accurately determines AAV titer and Empty/Full Ratios in purified or crude samples.
Biolayer Interferometry (BLI) is an established method that is traditionally used to study biomolecular interactions. This label-free technique measures the binding of biomolecules to the tip of a biosensor in real-time to determine kinetic rates of interaction pairs or to quantify biomolecular samples. The newest generation of AAV biosensors from Gator Bio opens up this application to the field of viral vectors and enables accurate and reliable quantitation of AAV total capsid titer. With the AAVX and AAV9 biosensors and the new high-sensitivity kits, Gator covers AAV serotypes 1 – 10 with a dynamic range from 5E+6 to 1E+13 vp/ml and a CV of <10%.
The new Empty/Full Ratio Kit is a practical and cost-effective solution for a challenging step in AAV development. With this new tool, the Empty/Full ratio of AAV samples can be determined after purification or in crude extracts with a dynamic range from 5% - 100% Full and 10% Resolution. A throughput of 8 samples in 40 minutes and a minimal sample volume of only 40 µl makes this kit not only a valuable asset in research, but also for upstream and downstream processes. Gator Bios AAV portfolio offers a single flexible technique with multiple applications in research and development of viral vectors that offer high accuracy, short assay times and a wide dynamic range.
Discover our portfolio of rapid and easy AAV Analytics solutions!
Great flexibility
Consistent AAV analytics during the manufacturing process is challenging due to a lack of standardized methods that can cover all process steps. Biolayer Interferometry (BLI) offers a flexible technique with various applications throughout the process including capsid titer, empty/full ratio determination and Tab and Nab assays. Gator Bio's AAV analytics solutions offer several advantages that make them suitable for integration into manufacturing processes from start to finish: crude sample compatibility, high sensitivity, accuracy, robustness, automation, and reduced hands-on time. With Gator Bio's AAV analytics solutions, you can expect reliable and accurate data to optimize your processes and produce high-quality products. By leveraging the flexibility and robustness of BLI, you can now streamline your AAV analytics and maintain consistency throughout the manufacturing process.
High accuracy quantitations
Gator Bio directly detects binding of biomolecules to the sensor surface in real-time, without the need for additional labels, antibodies or other indirect detection methods. Together with the new generation of precoated AAV biosensors, the high sensitivity of BLI makes the accuracy of Gator Bio superior to ELISA.
Short assay time
Measure up to 8 samples in parallel within 2 minutes. With minimal preparation time, the Gator Bio system can quantify 96 samples in under 30 minutes. The straight forward data analysis in the integrated software enables you to get results immediately after the assay.
Wide dynamic range
There is no need to dilute your samples, no matter if you are in early growth, purification or QC step. This reduces the hands-on time and eliminates errors from the sample dilution steps.
Empty/Full Ratio workflow
The approach involves three steps:
1. AAV capture on AAVX or AAV9 probes
2. Transfer and lysis of AAV particles to release the ssDNA
3. Quantitation of released ssDNA with ssDNA probes
ssDNA measurement
The figure shows ssDNA binding signal for lysed AAV2 capsids at the same concentration. The ssDNA signals are proportional to the corresponding Full Ratio of the sample.
Additional Features
- Robust: AAV titer and Empty/Full ratio can be determined in crude extracts, cell medium or purified samples.
- Low maintenance: Probes and sample plates are disposable. There is no need for extensive cleaning protocols or instrument maintenance.
- Cost effective: Automatic sensor regeneration keeps the assay cost low.
- Little material needed: Measurements can be performed with a minimal sample volume of only 40 µl.
- Low sample consumption: The Measurement is non-invasive. Precious sample material can be recovered after quantitation.
- User friendly: The intuitive software enables easy assay setup and analysis.
For more information, see the Application Note: Comparison of Gator® BLI and Progen ELISA for Quantitation of AAV Serotypes
Next generation of Biolayer Interferometry (BLI) Systems from Gator Bio
GatorPrime
Next Gen label-free Biolayer interferometry (BLI) instrument
The GatorPrime is our entry level instrument that brings next generation BLI technology into your lab. This versatile 8-channel instrument uses our variety of new high-performance biosensors and is ideal for groups that are working on a budget.
GatorPlus
Next gen label-free BLI instrument with high sensitivity
The Gator Plus is our advanced 8-channel system that provides you with the additional option to use 384 well plates. Increase your run times and significantly reduce your sample volume while still getting the same high-quality data sets from our innovative biosensors.
GatorPro
The most powerful BLI instrument in the world
The Gator Pro is our 32-channel system that puts high-throughput to the next level. Equipped to handle 3 x 384-well plates and ready for automation. Finish your 32 x 32 Epitope binning experiments in just 8 hours without suffering a loss in data quality.
