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SuperFlo® Wedge Chromatography Column

Sepragen developed the SuperFlo® Wedge column in order to simplify method development and the selection of the proper size of a SuperFlo production column. A Wedge column is a cut out version of a  SuperFlo column similar to a pie slice. The Wedge column provides identical bed-height (10, 15 or 20 cm) and surface area ratio of outer and inner frit as a SuperFlo production column. This allows to reliably simulate the flow characteristics of a production column. The Wedge column is packed with a slurry of your column resin similar to a standard SuperFlo column.

A method developed on a Wedge-column can easily be transferred to a production scale SuperFlo column. The developed gradient remains the same on the production column. It is only necessary to adjust the flow rates according to the volume ratio between production and Wedge column. For example: A 100 mL Wedge is used for method development and it should be transferred to a 10 L SuperFlo column. The ratio then is 100 (= 10 L/ 0.1 L). Assuming that the separation on the Wedge is performed at a flow of 30 mL/min, the separation on the 10 L SuperFlo is achieved under the same gradient conditions at 3 L/ min (= 30 mL x 100). SuperFlo Wedge allows the production method development on a small scale, saving time and costs for resin and buffer.

Technical Service


During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.

Detailed information about our support options can be found here.

Sepragen® Corporation

The Sepragen Corporation is based in Union City, California, and provides break-through products and processes to the biotechnology and nutraceutical industries for their process development and manufacturing needs. The company's products enable their customers several fold higher productivity in a smaller footprint, ease of use and rapid, predictable scale up of processes from the laboratory to production. This results in lower capital outlay, lower cost of goods and rapid time to market. All products are designed and manufactured to the highest standards of quality and with the standards of traceability and cGMP compliance.

More about Sepragen: www.sepragen.com

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131 employees
10 locations in europe
31 years of experience