NanoCellect® Biomedical Inc.
WOLF® G2 Cell Sorter
Gentle Benchtop Microfluidic Cell Sorting
The WOLF® G2 Cell Sorter allows for gentle analysis, sorting and plating of cells based on microfluidic technology. By using sterile, disposable fluidics, system clean-up becomes fast and cross contamination free. The WOLF G2 has 2 co-linear lasers and 7-9 fluorescent channels. Single-cell sorting can be completed in 96- or 384-well plates with the N1 Single-Cell Dispenser.
- Compact, robust and portable format for benchtop use
- Sterile, disposable fluidics for rapid clean-up without sample carryover
- Two-way sorting by scattering and up to nine emission colours (2 lasers)
- Low pressure system (<2 psi)
- Easy-to-use software
- Sample Aliquotting
- Sample Analysis
- Cell Culture
- Cell sorting
- Cell Biology
- 3D Cell Culture
- Alternative proteins
- Cultured meat
- Belgium
- Denmark
- Germany
- France
- Ireland
- Island
- Luxembourg
- Netherlands
- Austria
- Sweden
WOLF® G2 Cell Sorter – Gentle microfluidic cell sorting
The new WOLF® G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors, while maintaining simple workflows for either bulk sorting or single-cell dispensing. Whether you are focused on biopharma, genomics, or basic research, NanoCellect® delivers sterile, portable, intuitive solutions for gently sorting samples. Three laser combinations are available: 488/405 nm, 488/561 nm and 488/637 nm. The NanoCellect® WOLF® G2 uses a patented, microfluidic-based technology with robust laser-detection and easy-to-use WOLFviewer software. This in combination with the N1 Single Cell Dispenser allows higher cell viability including the opportunity to use sorted cells for further experiments. Cell sorting made easy.
- The 2-laser WOLF® G2 is available in 3 configurations and works with 7-9 fluorescent colours.
- The small size of WOLF® G2 makes your cell sorting flexible. It actually fits into a sterile hood.
- Sterile cartridges containing all the microfluidics is a warranty for contamination free experiments.
- The sample pressure of less than 2 psi makes the sorting most gentle.
- The optional N1 Single Cell Dispenser can dispense your cells in 96- or 384-well plates.
- Temperature-sensitive samples benefit from the optional Chiller-Stirrer, which keeps them at the desired temperature during the sorting process. In addition to the cooling mechanism, the CS1 Chiller-Stirrer can provide a stirring function for samples that settle quickly or aggregate.
Nanocellect | WOLF G2 Cell Sorter | Microfluidic Benchtop Cell Sorter
Technical data
Fluidics | |
Sample Input | 1.5 and 5.0 mL tubes |
Sheath Input | 50 mL Conical Tubes |
Sheath Fluid | PBS or Buffer of Choice |
Sheath Fluid Usage | 7.5 mL/Hour |
Sample Flow Rate | 24 ul/Minute |
Sheath Flow Rate | 160 uL/Minute |
Dead Volume | 50 uL |
Minimum Sample Volume | 150 uL |
Tubing Diameter (Inner) | 200 to 500 um |
Flow Cell | 200 x 70 um |
Sample Pressure | < 2psi |
| |
Excitation | 488 nm & 637 nm or 488 nm & 561 nm or 405 nm & 488 nm |
Laser Profile | 25 x 27 µm |
Scatter Detection | Forward (0 Degrees, +/-15) ; Back (180 Degrees, +/-15) |
Emission Detection | See WOLF G2 Configuration Guide |
Optical Alignment | Fixed alignment, no customer maintenance required |
| |
Scatter sensitivity | < 1.5 μm by FSC or BSC |
Scatter Resolution | Resolves lymphocytes, monocytes, and granulocytes |
Fluorescence sensitivity | < 200 MESF FITC (using 488 nm laser) < 250 MESF PE (using 561 nm laser) |
Fluorescence Resolution | 8 peak separation with SPHERO™️ Rainbow Calibration Particles |
Analysis Speed | > 5,000 events/second |
Sorting | 1- and 2-way |
Back-to-back Sorting Speed | 200 events per second |
Absolute Counts | Yes |
Volumetric Analysis | Yes |
Warm-up Time | Less than 1 minute |
Sorting Purity | Up to 99% purity |
Sorting Output | 1.5 mL and 5.0 mL, 96- and 384-well plates |
| |
WOLF Dimensions | 14.8 H x 18.0 W x 13.6 D in (37.6 H x 45.8 W x 34.5 D cm) |
WOLF Weight | 54 lbs (24.5 kg) |
WOLF Electrical | AC Input: 100-240 V, 50/60 Hz, 0.5 A |
N1 Dimensions | 8.42 W x 6.5 H x 8.34 D in (21.4 x 16.5 x 20.9) |
N1 Weight | 5.5 lbs / 2.5 kg |
N1 Electrical | DC Input: 24 V 1A |
Consumables
NC-170111 | Calibration Beads |
NC-150411 | Cartridge single cell sort |
NC-150410 | Cartridge bulk sort |
NC-170150 | Rainbow Beads 9 peak |
Accessories
NC-13020 | Chiller-Stirrer |
Art.-Nr. | N1 Pro Single Cell Dispenser |
Technical Service
During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.
Detailed information about our support options can be found here.
NanoCellect was founded in 2009 as a spinout from UCSD and dedicated 6 years developing the foundation of the WOLF’s technology before introducing the WOLF to early adopters in 2016. The company’s mission is to facilitate breakthrough biomedical discoveries by making cell analysis and sorting technology more portable, affordable, and easier to use. Their microfluidic flow cytometry platforms enable biomedical scientists to analyze and sort cells required for drug discovery, single cell-omics, cloning, and basic research.
More about NanoCellect: https://nanocellect.com
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