WOLF® G2 Cell Sorter – Gentle microfluidic cell sorting

The new WOLF® G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors, while maintaining simple workflows for either bulk sorting or single-cell dispensing. Whether you are focused on biopharma, genomics, or basic research, NanoCellect® delivers sterile, portable, intuitive solutions for gently sorting samples. Three laser combinations are available: 488/405 nm, 488/561 nm and 488/637 nm. The NanoCellect® WOLF® G2 uses a patented, microfluidic-based technology with robust laser-detection and easy-to-use WOLFviewer software. This in combination with the N1 Single Cell Dispenser allows higher cell viability including the opportunity to use sorted cells for further experiments. Cell sorting made easy.

  • The 2-laser WOLF® G2 is available in 3 configurations and works with 7-9 fluorescent colours.
  • The small size of WOLF® G2 makes your cell sorting flexible. It actually fits into a sterile hood.
  • Sterile cartridges containing all the microfluidics is a warranty for contamination free experiments.
  • The sample pressure of less than 2 psi makes the sorting most gentle.
  • The optional N1 Single Cell Dispenser can dispense your cells in 96- or 384-well plates.
  • Temperature-sensitive samples benefit from the optional Chiller-Stirrer, which keeps them at the desired temperature during the sorting process. In addition to the cooling mechanism, the CS1 Chiller-Stirrer can provide a stirring function for samples that settle quickly or aggregate.

Nanocellect | WOLF G2 Cell Sorter | Microfluidic Benchtop Cell Sorter

Technical data




Sample Input 1.5 and 5.0 mL tubes
Sheath Input 50 mL Conical Tubes
Sheath Fluid PBS or Buffer of Choice
Sheath Fluid Usage 7.5 mL/Hour
Sample Flow Rate 24 ul/Minute
Sheath Flow Rate 160 uL/Minute
Dead Volume 50 uL
Minimum Sample Volume 150 uL
Tubing Diameter (Inner) 200 to 500 um
Flow Cell 200 x 70 um
Sample Pressure < 2psi


Excitation 488 nm & 637 nm or 488 nm & 561 nm or 405 nm & 488 nm
Laser Profile 25 x 27 µm
Scatter Detection Forward (0 Degrees, +/-15) ; Back (180 Degrees, +/-15)
Emission Detection See WOLF G2 Configuration Guide
Optical Alignment Fixed alignment, no customer maintenance required


Scatter sensitivity < 1.5 μm by FSC or BSC
Scatter Resolution Resolves lymphocytes, monocytes, and granulocytes
Fluorescence sensitivity < 200 MESF FITC (using 488 nm laser)
< 250 MESF PE (using 561 nm laser)
Fluorescence Resolution 8 peak separation with SPHERO™️ Rainbow Calibration Particles
Analysis Speed > 5,000 events/second
Sorting 1- and 2-way
Back-to-back Sorting Speed 200 events per second
Absolute Counts Yes
Volumetric Analysis Yes
Warm-up Time Less than 1 minute
Sorting Purity Up to 99% purity
Sorting Output 1.5 mL and 5.0 mL, 96- and 384-well plates

Physical Specifications

WOLF Dimensions 14.8 H x 18.0 W x 13.6 D in (37.6 H x 45.8 W x 34.5 D cm)
WOLF Weight 54 lbs (24.5 kg)
WOLF Electrical AC Input: 100-240 V, 50/60 Hz, 0.5 A
N1 Dimensions 8.42 W x 6.5 H x 8.34 D in (21.4 x 16.5 x 20.9)
N1 Weight 5.5 lbs / 2.5 kg
N1 Electrical DC Input: 24 V 1A








NC-170111 Calibration Beads
NC-150411 Cartridge single cell sort
NC-150410 Cartridge bulk sort
NC-170150 Rainbow Beads 9 peak



NC-13020 Chiller-Stirrer
Art.-Nr. N1 Pro Single Cell Dispenser




Technical Service

During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.

Detailed information about our support options can be found here.

NanoCellect Biomedical Inc.
NanoCellect® Biomedical Inc.

NanoCellect was founded in 2009 as a spinout from UCSD and dedicated 6 years developing the foundation of the WOLF’s technology before introducing the WOLF to early adopters in 2016. The company’s mission is to facilitate breakthrough biomedical discoveries by making cell analysis and sorting technology more portable, affordable, and easier to use. Their microfluidic flow cytometry platforms enable biomedical scientists to analyze and sort cells required for drug discovery, single cell-omics, cloning, and basic research.

More about NanoCellect: https://nanocellect.com

125 employees
10 locations in europe
32 years of experience
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