Gator Bio
GatorPlus BLI System
Next gen label-free BLI instrument with high sensitivity
GatorPlus is a next generation Biolayer Interferometry (BLI) system. Among the applications are label-free quantitation and kinetic analysis of antibodies, AAV, proteins, peptides, nucleic acids, aptamers, liposomes, viruses, small molecules and epitope binning/mapping. The system uses the 384-well plate format and offers best-in-class baseline stability for higher sensitivity.
More about Next-gen Biolayer Interferometry
- 8-channel system
- 384-well plate format
- Measure 1 – 456 samples per run
- Wide range of biosensors with improved surface chemistry
- Automated biosensor loading and regeneration
- Intuitive software with integrated assay setup, data acquisition and analysis
- Upstream
- Protein Analysis
- Automation
- Biopharmaceutical Quality Control
- Microbial Fermentation
- Good manufacturing practice (GMP)
- Cell Culture
- AAV (Adeno-associated viruses)
- Biotechnology
- Belgium
- Denmark
- Germany
- Netherlands
- Austria
- Sweden
- France
- Great Britain
- Ireland
- Luxembourg
- Switzerland
GatorPlus – Biolayer Interferometry (BLI) System
The GatorPlus from Gator Bio is a label-free analysis instrument based on the biolayer interferometry (BLI) technology. It´s main applications are biomolecular quantitation, determination of intermolecular binding kinetics and epitope binning. This 8-channel system can quantify 8 samples in 60 second and 384 samples in less than 120 minutes. The real-time detection of binding kinetics allows for the determination of kon, koff and Kd of up to 192 samples in one experiment. A double thermal insulation of the optical bench leads to best in-class baseline stability, which improves the sensitivity while measuring long off-rates of tight binding interactions. The 384-well plate format enables 16x16 epitope binning experiments in the classical sandwich or tandem assay. The GatorPlus is a fluidic-free benchtop instrument that is robust enough to handle everything from purified samples to crude extracts or serum.
The integrated software allows for intuitive assay setup, data acquisition and analysis. Gator Bio also offers a new generation of biosensors with improved surface chemistry for enhanced sensitivity, binding capacity, robustness and regenerative capability. A wide choice of pre-coated biosensors allows for the quantitation and kinetic analysis of proteins, antibodies, peptides, nucleic acids, aptamers, liposomes, AAV and small molecules. The GatorPlus is our advanced BLI instrument that provides a powerful and versatile tool to scientists from drug discovery to therapeutics manufacturing QC.
- Regenerable biosensors to save time and cost per assay
- Best in-class baseline stability to measure long off-rates of tight binders
- Quantify 384 samples in under 120 minutes
- Determine kon, koff and Kd of up to 192 samples in one run
- 16x16 Epitope binning
- Can be used with crude extracts, serum or plasma
- Supports 21 CFR part 11 (GMP, GLP)
Discover the next generation of BLI technology!
Gator Bio 96-well flat plate
The Gator Bio® BLI 96-Flat Plate is a black polypropylene 96-well flat-bottom plate that meets the Standard Society for Biomolecular Screening (SBS) specifications. It is designed for use in Bio-Layer Interferometry (BLI) experiments that measures biomolecular interactions of proteins, peptides, small molecules, and viruses. The BLI 96-Flat plate can be utilized on Gator Bio® instruments in an array of applications, as well as other BLI platforms that accommodate a 96-well microplate format.
Gator Bio® BLI 96-well plates are available by us - please feel free to ask us!
The history of BLI and introduction of the Next Gen Gator BLI system
Gator Bio Flex SA Kit Video
Gator Bio BPIWest AAVX Part1
Gator Bio BPIWest AAVX Part2
Technical data
| Detection technology: | Biolayer Interferometry |
| Analysis types: | Quantitation, Yes/No Binding, Kinetics, Affinity |
| Sample types: | Proteins, Antibodies, Peptides, Nucleid Acids, Liposomes, Small Molecules, AAV |
| Number of Channels: | 8 |
| Data Aquisition rate: | 2 Hz, 5 Hz, 10 Hz |
| Plate format: | 1 x 96- or 1 x 384-well plate & 1x 96-well max plate |
| Max samples per run: | 456 |
| Quantitation throughput: | 384 samples in less than 2 hours |
| Kinetics per run: | 192 |
| Epitope binning: | 16 x 16 |
| Minimum sample volume: | 40 µl |
| Baseline noise: | ≤ 4 pm (RMS) |
| Baseline drift: | ≤ 0.1 nm/hour |
| Association rate kon: | 101 – 107 M-1 s-1 |
| Dissociation rate koff: | 10-6 – 10-1 s-1 |
| Affinity constant Kd: | 10 pm – 1 mM |
| Orbitaler Fluss: | 100 – 2000 rpm |
| Temperature: | Ambient to 40°C |
| Dimensions: | 63 cm x 73 cm x 44 cm (H x W x D) |
| Weight: | 50 kg |
| Electrical: | 110-220 V |
| Power consumption: | 200 W |
| Safety Standards: | CE, CSA, FCC |
Downloads
Consumables
Working with Antibodies
| Probe | Dynamic Range | Regeneration |
| Protein A | 0.02 – 2000 µg/ml | Yes |
| Protein G | 0.02 – 2000 µg/ml | Yes |
| Protein L | 0.02 – 2000 µg/ml | Yes |
| Anti-Human IgG Fc Gen II | 0.3 – 6000 µg/ml | Yes |
| Anti-Human FAB | 0.3 – 3000 µg/ml | Yes |
| Anti-Mouse IgG Fc | 0.05 – 100 µg/ml | Yes |
Working with AAV
| Probe | Serotypes | Dynamic Range | Regeneration |
| AAVX | AAV 1-8 and 10 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| AAV9 | AAV 9 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| High sensitivity AAV | AAV 1-8 | 5 x 106 – 5 x 1010 vp/mL | No |
Working with other biomolecules
Probe | Regeneration |
No | |
Yes | |
Yes | |
No | |
No | |
Yes | |
Yes | |
No |
Technical Service
During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.
Detailed information about our support options can be found here.
Gator Bio is a life sciences company providing bioanalytical systems to accelerate the development of therapeutics and diagnostics. Gator Bio along with its sister company ET Healthcare are part of Access Medical Systems. The Gator instruments and biosensors enable real-time analysis of biomolecular interactions providing information on affinity, kinetics, concentration and epitope binning, etc. Most importantly, Gator Bio's analytical capabilities enable better and faster characterization of drug candidates, thus providing greater value in drug development applications where existing methods have limitations in throughput, performance, and cost. The company is headquartered in Palo Alto, California with facilities in Shanghai and Suzhou, China.
For more information, please visit www.gatorbio.com
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