Loading...

GatorPlus | Biolayer Interferometry (BLI) System

The GatorPlus from Gator Bio is a label-free analysis instrument based on the biolayer interferometry (BLI) technology. It´s main applications are biomolecular quantitation, determination of intermolecular binding kinetics and epitope binning. This 8-channel system can quantify 8 samples in 60 second and 384 samples in less than 120 minutes. The real-time detection of binding kinetics allows for the determination of kon, koff and KD of up to 192 samples in one experiment. A double thermal insulation of the optical bench leads to best in-class baseline stability, which improves the sensitivity while measuring long off-rates of tight binding interactions. The 384-well plate format enables 16x16 epitope binning experiments in the classical sandwich or tandem assay. The GatorPlus is a fluidic-free benchtop instrument that is robust enough to handle everything from purified samples to crude extracts or serum.

The integrated software allows for intuitive assay setup, data acquisition and analysis. Gator Bio also offers a new generation of biosensors with improved surface chemistry for enhanced sensitivity, binding capacity, robustness and regenerative capability. A wide choice of pre-coated biosensors allows for the quantitation and kinetic analysis of proteins, antibodies, peptides, nucleic acids, aptamers, liposomes, AAV and small molecules. The GatorPlus is our advanced BLI instrument that provides a powerful and versatile tool to scientists from drug discovery to therapeutics manufacturing QC.

  • Regenerable biosensors to save time and cost per assay
  • Best in-class baseline stability to measure long off-rates of tight binders
  • Quantify 384 samples in under 120 minutes
  • Determine kon, koff and KD of up to 192 samples in one run
  • 16x16 Epitope binning
  • Can be used with crude extracts, serum or plasma
  • Supports 21 CFR part 11 (GMP, GLP)

Applications

Quantitation

Fast determination of biomolecular concentration with a wide dynamic range.

Learn more

Kinetics

Determine kon, koff and Kd of interaction pairs in one experiment.

For more information, see the Application Note: Biotinylation Protocol for Protein Immobilization on Streptavidin BLI Probes

Download Biotinylation Protocol Application Note

Learn more

Gene therapy

Quantify Adeno Associated Virus (AAV) total capsid titer from crude extracts.

For more information, see the Application Note: Comparison of Gator Bio BLI and Progen ELISA for Quatitation of AAV Serotypes

Download AAV Quantitation Application Note

Learn more

Immunotherapy

Support your monoclonal antibody development from discovery to manufacturing.  

For more information, see the Application Note: Accelerating Antibody Discover through Gator Bio BLI Biosensor Technology

Download Antibody Solutions Application Note

Learn more

Small Molecules

The SMAP biosensor can detect binding of small molecules down to 150 Da.

Learn more

Epitope Binning

Investigate crude supernatant to save time in the earliest drug discovery phases.

Learn more

The history of BLI and introduction of the Next Gen Gator BLI system

Gator Bio Flex SA Kit Video

Gator Bio BPIWest AAVX Part1

Gator Bio BPIWest AAVX Part2

Technical data

 

 

Detection: Biolayer interferometry
Number of Spectrometers/channels: 8/8
Data aquisition rate: 2 Hz, 5 Hz, 10 Hz
Orbital flow: 100 – 2000 rpm
Dimensions:  63 cm x 67 cm x 44 cm (HxWxD)
Weight: 50 kg
Electrical:  110-220 V
Power consumption:  200W
Safety Standards: CE, CSA, FCC

 

Consumables

 

Working with Antibodies

Probe Dynamic Range Regeneration
Protein A 0.02 – 2000 µg/ml Yes
Protein G 0.02 – 2000 µg/ml Yes
Protein L 0.02 – 2000 µg/ml Yes
Anti-Human IgG Fc Gen II 0.3 – 6000 µg/ml Yes
Anti-Human FAB 0.3 – 3000 µg/ml Yes
Anti-Mouse IgG Fc 0.05 – 100 µg/ml Yes

 

 

Working with AAV

Probe Serotypes Dynamic Range Regeneration
AAVX AAV 1-8 and 10 1 x 10– 1 x 1013 vp/mL  Yes
AAV9 AAV 9 1 x 109 – 1 x 1013 vp/mL  Yes
High sensitivity AAV AAV 1-8 5 x 106 – 5 x 1010 vp/mL  No

 

 

Working with other biomolecules

Probe

Regeneration

Streptavidin

No

Streptavidin FLEX

Yes

Aminopropylsilane

No

Amine Reactive

No

Anti-His

Yes

Ni-NTA

Yes

Small Molecule Analysis

No

 

 

 

Technical Service


During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.

Detailed information about our support options can be found here.

Gator Bio

Gator Bio is a life sciences company providing bioanalytical systems to accelerate the development of therapeutics and diagnostics. Gator Bio along with its sister company ET Healthcare are part of Access Medical Systems. The Gator instruments and biosensors enable real-time analysis of biomolecular interactions providing information on affinity, kinetics, concentration and epitope binning, etc. Most importantly, Gator Bio's analytical capabilities enable better and faster characterization of drug candidates, thus providing greater value in drug development applications where existing methods have limitations in throughput, performance, and cost. The company is headquartered in Palo Alto, California with facilities in Shanghai and Suzhou, China.

For more information, please visit www.gatorbio.com

Request

Request product info

Your contact data

* required infos

Your contact person

No person found
131 employees
10 locations in europe
31 years of experience