Gator Bio
GatorPrime BLI System
Next Gen label-free Biolayer interferometry (BLI) instrument
GatorPrime is a next generation Biolayer Interferometry (BLI) system. Among the applications are label-free quantitation and kinetic analysis of antibodies, AAV, proteins, peptides, nucleic acids, aptamers, liposomes, viruses, small molecules and epitope binning/mapping. This system uses a variety of new biosensors to make assay setup and performance as fast and easy and possible.
- 8-channel system
- 96-well format
- Measure 1 – 168 samples per run
- Wide range of biosensors with improved surface chemistry
- Automated biosensor loading and regeneration
- Intuitive software with integrated assay setup, data acquisition and analysis
- Automation
- Biopharmaceutical Quality Control
- Microbial Fermentation
- Good manufacturing practice (GMP)
- Protein Analysis
- Upstream
- Cell Culture
- AAV
- Biotechnology
- Belgium
- Denmark
- Germany
- Netherlands
- Austria
- Sweden
- France
- Great Britain
- Ireland
- Luxembourg
- Switzerland
GatorPrime | Biolayer Interferometry (BLI) system
The GatorPrime from Gator Bio is a label-free analysis instrument based on the biolayer interferometry (BLI) technology. It´s main applications are biomolecular quantitation, determination of intermolecular binding kinetics and epitope binning. This 8-channel system can quantify 8 samples in 60 second and 96 samples in less than 30 minutes. The real-time detection of binding kinetics allows for the determination of kon, koff and KD of up to 72 samples in one experiment. An extra thermal insulation of the optical bench leads to improved baseline stability, which increases the sensitivity while measuring long off-rates of tight binding interactions. The 96-well plate format enables 10x10 epitope binning experiments in the classical sandwich or tandem assay. The GatorPrime is a fluidic-free benchtop instrument that is robust enough to handle everything from purified samples to crude extracts or serum. The integrated software allows for intuitive assay setup, data acquisition and analysis. Gator Bio also offers a new generation of biosensors with improved surface chemistry for enhanced sensitivity, binding capacity, robustness and regenerative capability.
A wide choice of pre-coated biosensors allows for the quantitation and kinetic analysis of proteins, antibodies, peptides, nucleic acids, aptamers, liposomes, AAV and small molecules. The GatorPrime is our entry level BLI instrument that provides a powerful and versatile tool to scientists from drug discovery to therapeutics manufacturing QC and in academia.
- Regenerable biosensors to save time and cost per assay
- Improved baseline stability to measure long off-rates of tight binders
- Quantify 96 samples in under 30 minutes
- Determine kon, koff and KD of up to 72 samples in one run
- 10x10 Epitope binning
- Can be used with crude extracts, serum or plasma
- Supports 21 CFR part 11 (GMP, GLP)
Applications
Kinetics
Determine kon, koff and Kd of interaction pairs in one experiment.
For more information, see the Application Note: Biotinylation Protocol for Protein Immobilization on Streptavidin BLI Probes
Immunotherapy
Support your monoclonal antibody development from discovery to manufacturing.
For more information, see the Application Note: Accelerating Antibody Discover through Gator Bio BLI Biosensor Technology
Gene therapy
Quantify Adeno Associated Virus (AAV) total capsid titer from crude extracts.
For more information, see the Application Note: Comparison of Gator Bio BLI and Progen ELISA for Quatitation of AAV Serotypes
The history of BLI and introduction of the Next Gen Gator BLI system
Gator Bio Flex SA Kit Video
Gator Bio BPIWest AAVX Part1
Gator Bio BPIWest AAVX Part2
Technical data
| GENERAL | |
| Detection: | Biolayer interferometry |
| Number of channels: | 8 |
| Data collection rate : | 2 Hz, 5 Hz, 10 Hz |
| Orbital flow: | 0 – 1,500 rpm |
| Dimensions: | 460 mm x 670 mm x 320 mm (H x W x D) |
| Weight: | 30 kg |
| Electrical: | 110 V - 220 V 5A |
| Power: | 200 W |
| KINETICS | |
| Analysis time: | Real-time kinetic binding from 1 minute to 4 hours |
| Association rate kon: | kon 101 - 106 M-1 /s |
| Dissociation rate koff: | koff 10-6 - 10-1 /s |
| Affinity constant KD: | KD 10 pM - 1 mM |
| Binding curve baseline nois: | <3 pm (RMS) |
| Binding curve baseline drift: | <40 pm/hour (RMS) |
| ANALYSIS | |
| Sample types : | Proteins, antibodies, peptides, DNA, RNA, liposomes, cells, viruses, etc. |
| Work flow: | • 2 x 96-well plates, in tilted or flat position • Up to 8 samples in parallel • Up to 96 samples per 96-well plate |
| Types of analysis: | • Yes or no binding • Relative and absolute concentration • Kinetics and affinity |
| QUANTITATION | |
| Analysis time IgG-Concentration: | • in 30 seconds for 8 samples • <10 minutes for 96 samples |
| Linear range for Gator Protein A biosensor: | • 0,025 - 2.000 μg/mL |
Downloads
Consumables
Working with Antibodies
| Probe | Dynamic Range | Regeneration |
| Protein A | 0.02 – 2000 µg/ml | Yes |
| Protein G | 0.02 – 2000 µg/ml | Yes |
| Protein L | 0.02 – 2000 µg/ml | Yes |
| Anti-Human IgG Fc Gen II | 0.3 – 6000 µg/ml | Yes |
| Anti-Human FAB | 0.3 – 3000 µg/ml | Yes |
| Anti-Mouse IgG Fc | 0.05 – 100 µg/ml | Yes |
Working with AAV
| Probe | Serotypes | Dynamic Range | Regeneration |
| AAVX | AAV 1-8 and 10 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| AAV9 | AAV 9 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| High sensitivity AAV | AAV 1-8 | 5 x 106 – 5 x 1010 vp/mL | No |
Working with other biomolecules
Probe | Regeneration |
No | |
Yes | |
No | |
No | |
Yes | |
Yes | |
No |
Technical Service
During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.
Detailed information about our support options can be found here.
Gator Bio is a life sciences company providing bioanalytical systems to accelerate the development of therapeutics and diagnostics. Gator Bio along with its sister company ET Healthcare are part of Access Medical Systems. The Gator instruments and biosensors enable real-time analysis of biomolecular interactions providing information on affinity, kinetics, concentration and epitope binning, etc. Most importantly, Gator Bio's analytical capabilities enable better and faster characterization of drug candidates, thus providing greater value in drug development applications where existing methods have limitations in throughput, performance, and cost. The company is headquartered in Palo Alto, California with facilities in Shanghai and Suzhou, China.
For more information, please visit www.gatorbio.com
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