Gator Bio
GatorPro BLI System
The most powerful BLI instrument in the world
The GatorPro is the high-throughput Biolayer Interferometry (BLI) system of the next-generation. Its main applications are label-free quantitation and kinetic analysis of antibodies, Adeno-associated virus (AAV), proteins, peptides, nucleic acids, aptamers, lipid nanoparticles, viruses, small molecules and epitope binning/mapping. This versatile 32-channel system puts throughput to the next level. Equipped to handle 3 x 384-well plates simultaneously and ready for automation. It uses the established variety of next-generation biosensors to supercharge the performance of your BLI assays.
More about Next-gen Biolayer Interferometry
- 32-channel system
- Quantitate 1152 samples in 142 minutes
- Wide range of high sensitivity biosensors
- Automation compatible
- Intuitive data acquisition and analysis software
- Automation
- Biopharmaceutical Quality Control
- Microbial Fermentation
- Good manufacturing practice (GMP)
- Protein Analysis
- Upstream
- Cell Culture
- AAV (Adeno-associated viruses)
- Biotechnology
- Belgium
- Denmark
- Germany
- Netherlands
- Austria
- Sweden
- France
- Great Britain
- Ireland
- Luxembourg
- Switzerland
GatorPro – Biolayer Interferometry (BLI) system for high throughput
The GatorPro from Gator Bio is a label-free analysis instrument based on the biolayer interferometry (BLI) technology. It´s main applications are biomolecular quantitation, determination of intermolecular binding kinetics and epitope binning. This 32-channel system can quantify 32 protein or AAV samples in 120 second or up to 1152 samples in 142 minutes. The real-time detection of binding kinetics allows for the determination of kon, koff and KD of up to 32 interaction pairs in parallel. Improved baseline stability allows for extra-long measurements of the dissociation step to precisely determine off-rates of tight binding interactions. The 3 x 384-well plate setup enables 32 x 32 epitope binning experiments in the classical sandwich or tandem assay and the automation compatibility allows for extra-long walk away times.
GatorPro is fluidics-free and robust enough to handle everything from purified samples to crude extracts or serum, which makes it easily integrated into every step of your process. The included software allows for intuitive assay setup, data acquisition and analysis that even unexperienced users can use easily. Additionally, Gator Bio also offers a new generation of biosensors with improved surface chemistry for enhanced sensitivity, binding capacity, robustness and regenerative capability.
A wide choice of pre-coated biosensors allows for the quantitation and kinetic analysis of proteins, antibodies, peptides, nucleic acids, aptamers, lipid nanoparticles, AAV and small molecules. The GatorPro is our most powerful BLI instrument that pairs high throughput with state-of-the-art sensitivity. This makes it a versatile tool for scientists from early drug discovery to therapeutics manufacturing QC.
• Regenerable biosensors to save time and cost per assay
• Improved baseline stability for prolonged off-rate measurements of tight binders
• Determine kon, koff and KD of up to 32 samples in parallel
• 32 x 32 Epitope binning
• Works with crude extracts, serum or plasma
• Supports 21 CFR part 11 (GMP, GLP)
Discover the next generation of BLI technology!
Applications
Kinetics
Determine kon, koff and Kd of interaction pairs in one experiment.
For more information, see the Application Note: Biotinylation Protocol for Protein Immobilization on Streptavidin BLI Probes
Adeno associated virus (AAV)
Quantify Adeno Associated Virus (AAV) total capsid titer from crude extracts.
For more information, see the Application Note: Comparison of Gator Bio BLI and Progen ELISA for Quatitation of AAV Serotypes
Antibody development
Support your monoclonal antibody development from discovery to manufacturing.
For more information, see the Application Note: Accelerating Antibody Discover through Gator Bio BLI Biosensor Technology
Gator Bio 96-well flat plates
The Gator Bio® BLI 96-Flat Plate is a black polypropylene 96-well flat-bottom plate that meets the Standard Society for Biomolecular Screening (SBS) specifications. It is designed for use in Bio-Layer Interferometry (BLI) experiments that measures biomolecular interactions of proteins, peptides, small molecules, and viruses. The BLI 96-Flat plate can be utilized on Gator Bio® instruments in an array of applications, as well as other BLI platforms that accommodate a 96-well microplate format.
Gator Bio® BLI 96-well plates are available by us - please feel free to ask us!
Technical data
| GENERAL | |
| Detection: | Biolayer interferometry |
| Number of channels: | 32 |
| Data collection rate : | 2 Hz, 5 Hz, 10 Hz |
| Orbital flow: | 0 – 2,000 rpm |
| Dimensions: | 840 mm x 1140 mm x 770 mm (H x W x D) |
| Weight: | 220 kg |
| Electrical: | 110 V - 220 V 5A |
| Power: | 200 W |
| KINETICS | |
| Analysis time: | Real-time kinetic binding from 1 minute to 4 hours |
| Association rate kon: | kon 101 - 107 M-1 /s-1 |
| Dissociation rate koff: | koff 10-6 - 10-1 /s |
| Affinity constant KD: | KD 10 pM - 1 mM |
| Binding curve baseline nois: | <4 pm (RMS) |
| Binding curve baseline drift: | <0.1 nm/hour (RMS) |
| ANALYSIS | |
| Sample types : | Proteins, antibodies, peptides, DNA, RNA, liposomes, cells, viruses, etc. |
| Work flow: | • 3x 384 or 96 well plates and 1x 96 well max plate • Up to 32 samples in parallel |
| Types of analysis: | • Yes or no binding • Relative and absolute concentration • Kinetics and affinity |
| QUANTITATION | |
| Analysis time IgG-Concentration: | • in 120 seconds for 32 samples • 1152 samples in 142 minutes |
| Linear range for Gator Protein A biosensor: | • 0,025 - 2.000 μg/mL |
Downloads
Consumables
Working with Antibodies
| Probe | Dynamic Range | Regeneration |
| Protein A | 0.02 – 2000 µg/ml | Yes |
| Protein G | 0.02 – 2000 µg/ml | Yes |
| Protein L | 0.02 – 2000 µg/ml | Yes |
| Anti-Human IgG Fc Gen II | 0.3 – 6000 µg/ml | Yes |
| Anti-Human FAB | 0.3 – 3000 µg/ml | Yes |
| Anti-Mouse IgG Fc | 0.05 – 100 µg/ml | Yes |
Working with AAV
| Probe | Serotypes | Dynamic Range | Regeneration |
| AAVX | AAV 1-8 and 10 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| AAV9 | AAV 9 | 1 x 109 – 1 x 1013 vp/mL | Yes |
| High sensitivity AAV | AAV 1-8 | 5 x 106 – 5 x 1010 vp/mL | No |
Working with other biomolecules
Probe | Regeneration |
No | |
Yes | |
Yes | |
No | |
No | |
Yes | |
Yes | |
No |
Technical Service
During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.
Detailed information about our support options can be found here.
Gator Bio is a life sciences company providing bioanalytical systems to accelerate the development of therapeutics and diagnostics. Gator Bio along with its sister company ET Healthcare are part of Access Medical Systems. The Gator instruments and biosensors enable real-time analysis of biomolecular interactions providing information on affinity, kinetics, concentration and epitope binning, etc. Most importantly, Gator Bio's analytical capabilities enable better and faster characterization of drug candidates, thus providing greater value in drug development applications where existing methods have limitations in throughput, performance, and cost. The company is headquartered in Palo Alto, California with facilities in Shanghai and Suzhou, China.
For more information, please visit www.gatorbio.com
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