The next generation of Biolayer Interferometry (BLI) technology Learn more
Gator Bio BLI system label free analysis
Gator Bio BLI system label free analysis

Improve your label-free analysis of biomolecular interactions with the newest innovations in BLI technology.

With the Gator Bio systems, the original inventors of the BLI technology introduce a new generation of more powerful, productive and cost-effective BLI instruments to the scientific community. The Gator Bio instruments offer an improved design, paired with the newest developments in biosensor technology. The improved thermal insulation of the optical bench leads to a more stable baseline, which helps while measuring long off-rates of tight binding interactions. A new generation of biosensors contain improved surface chemistry, which enhances their sensitivity, binding capacity, robustness and regeneration capability. The BLI technology from Gator Bio is flexible, highly-sensitive and accessible. From experiment to analysis, you can characterize the widest range of molecules on a single state-of-the-art platform - in a single run.

Key features of the Gator Bio system include:

  • Innovative Biosensors
  • Enhanced sensitivity
  • More stable baseline
  • Two-plate setup for increased sample capacity
  • Frequent releases of new and improved biosensors and kits


Accelerate your Antibody development Applications:

  • Rapid and easy quantitation of Antibody titer with wide dynamic range
  • High-throughput kinetic experiments to determine kon, koff and Kd of interaction pairs
  • 32 x 32 Epitope binning assay in 8 hours; Complete a 96 x 96 binning matrix in 5 days


Download Antibody Solution Application Note

Gator Bio BLI System 3D

More powerful

The next generation of BLI comes with the newest advancements in biosensor technology. The improved surface chemistry enhances the sensitivity of the sensors and increases the dynamic range in which they can measure. That means that you can quantify lower concentrations and resolve higher affinities with more precision. The prime example for this is the Small-Molecule-Analysis-Probe (SMAP), which redefines the limits of molecular weight that can be detected with BLI. Additionally, the research and development team at Gator Bio never stops to develop new and improved biosensors. This ensures, that you will be working with state-of-the-art technology for the long run.

first generation BLI system

Next geneartion BLI system

Kinetic characterization of Anti-fluoreszine and fluoreszine (332 Da). The innovative three-dimensional surface chemistry of the new SMAP biosensors drastically increases the binding capacity and thereby enhances sensitivity when working with biotinylated molecules.

Best-in-class baseline stability

Long-term baseline stability is a key element when measuring slow dissociation rates. Gator Bios improved thermal insulation of the optical bench greatly reduces background noise which results in a clearer signal. This makes it possible to extend the measurement time in the dissociation step and to precisely measure high affinity interactions up to picomolar range. Accelerate your antibody discovery by identifying strong candidates early and with high accuracy.



Kinetic characterization of TNF-α and anti-TNF-α. A clean signal with little noise and minimal baseline drift enables extended off-rate measurements to determine picomolar interactions with high precision.

Gator Bio BLI system: Best-in-class baseline stability

More productive

For Gator Bio, the user experience is as important as the technical advantages. The two-plate design opens up space for your important samples. The Max plate can be filled up with sensors, wash- and regeneration-steps, while the second plate remains completely free for up to 96 samples. New biosensor technologies like the Flex SA Kit, which lets you reactivate and reuse your streptavidin, let you get more experiments done with less probes and increased walk-away times. This is complemented by an integrated user-friendly software that even unexperienced lab members will learn to easily operate in no time.

Gator Bio FLEX SA Kit

Gator Bio FLEX SA Kit

Gator Bio BLI System time

Kinetic characterization of TNF-α and anti-TNF-α for over 10 reactivations. The FLEX SA Kit lets you reactivate your Streptavidin sensors so you get more experiments done with less probes. The days of single use biosensors are over.

Cost effective

Gator Bios patented biosensor surface chemistry is more robust than ever before. This increases their regeneration capability, so you can save on cost with each sensor. You´ll get twenty experiments done for the price of one. Gator Bio strives to make BLI accessible for as many researchers as possible. That’s why the Gator Bio instruments are very affordable, not only for industry, but also for academic labs.


Do you think a state-of-the-art BLI system is too expensive for your lab? You will be surprised! Ask us for a quote on the instruments.

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First generation BLI mFc

mFc Gator Bio BLI System

Regeneration profile: Concentration series of mouse IgG (10 µg/ml - 0.1 µg/ml) measured 20 times with sensor regeneration. The improved biosensor surface chemistry allows for more regeneration cycles without a loss in binding capacity.

Additional features

  • Wide dynamic range: The improved biosensor surface chemistry increases the concentration range for quantitations
  • Unique applications: Quantify your AAV samples with the new ready-to-use AAVX-probes
  • User friendly: The intuitive software enables fast and easy assay setup and analysis - even for users with little experience
  • Customer support: Gator Bio listens to customer feedback and incorporates it into future releases and updates
  • Future prove: The frequent releases of new and improved biosensors ensure that your assays are always performed with state-of-the-art technology

Gator Bio Software

Gator Bio AAV application BLI vs. ELISA

Determine AAV total Capsid Titer and Empty/Full Ratio with Biolayer Interferometry (BLI) technology from Gator Bio

Discover the flexible technique that accurately determines AAV titer and Empty/Full Ratios in purified or crude samples.

Biolayer Interferometry (BLI) is an established method that is traditionally used to study biomolecular interactions. This label-free technique measures the binding of biomolecules to the tip of a biosensor in real-time to determine kinetic rates of interaction pairs or to quantify biomolecular samples. The newest generation of AAV biosensors from Gator Bio opens up this application to the field of viral vectors and enables accurate and reliable quantitation of AAV total capsid titer. With the AAVX and AAV9 biosensors and the new high-sensitivity kits, Gator covers AAV serotypes 1 – 10 with a dynamic range from 5E+6 to 1E+13 vp/ml and a CV of <10%.

The new Empty/Full Ratio Kit is a practical and cost-effective solution for a challenging step in AAV development. With this new tool, the Empty/Full ratio of AAV samples can be determined after purification or in crude extracts with a dynamic range from 5% - 100% Full and 10% Resolution. A throughput of 8 samples in 40 minutes and a minimal sample volume of only 40 µl makes this kit not only a valuable asset in research, but also for upstream and downstream processes. Gator Bios AAV portfolio offers a single flexible technique with multiple applications in research and development of viral vectors that offer high accuracy, short assay times and a wide dynamic range.

Request a demo to convince yourself of the new AAV solutions from Gator Bio!

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Additional Features

  • Robust: AAV titer and Empty/Full ratio can be determined in crude extracts, cell medium or purified samples.
  • Low maintenance: Probes and sample plates are disposable. There is no need for extensive cleaning protocols or instrument maintenance.
  • Cost effective: Automatic sensor regeneration keeps the assay cost low.
  • Little material needed: Measurements can be performed with a minimal sample volume of only 40 µl.
  • Low sample consumption: The Measurement is non-invasive. Precious sample material can be recovered after quantitation.
  • User friendly: The intuitive software enables easy assay setup and analysis.

For more information, see the Application Note: Comparison of Gator® BLI and Progen ELISA for Quantitation of AAV Serotypes


Download AAV Quantitation Application Note

Next generation of Biolayer Interferometry (BLI) Systems from Gator Bio

Gator Bio GatorPrime BLI system for AAV



Next Gen label-free Biolayer interferometry (BLI) instrument

The GatorPrime is our entry level instrument that brings next generation BLI technology into your lab. This versatile 8-channel instrument uses our variety of new high-performance biosensors and is ideal for groups that are working on a budget.

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Gator Bio GatorPlus BLI system for AAV


Next gen label-free BLI instrument with high sensitivity

The Gator Plus is our advanced 8-channel system that provides you with the additional option to use 384 well plates. Increase your run times and significantly reduce your sample volume while still getting the same high-quality data sets from our innovative biosensors.

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The most powerful BLI instrument in the world

The Gator Pro is our 32-channel system that puts high-throughput to the next level. Equipped to handle 3 x 384-well plates and ready for automation. Finish your 32 x 32 Epitope binning experiments in just 8 hours without suffering a loss in data quality.


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Gator Bio BLI system overview


Do you have any questions? - Write to us. We will be glad to help you!

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