With the Gator Bio systems, the original inventors of the BLI technology introduce a new generation of more powerful, productive and cost-effective BLI instruments to the scientific community. The Gator Bio instruments offer an improved design, paired with the newest developments in biosensor technology. The improved thermal insulation of the optical bench leads to a more stable baseline, which helps while measuring long off-rates of tight binding interactions. A new generation of biosensors contain improved surface chemistry, which enhances their sensitivity, binding capacity, robustness and regeneration capability. The BLI technology from Gator Bio is flexible, highly-sensitive and accessible. From experiment to analysis, you can characterize the widest range of molecules on a single state-of-the-art platform - in a single run.
 

Key features of the Gator Bio system include:

• Innovative Biosensors
• Enhanced sensitivity
• More stable baseline
• Two-plate setup for increased sample capacity
• Frequent releases of new and improved biosensors and kits

Accelerate your Antibody development Applications:

• Rapid and easy quantitation of Antibody titer with wide dynamic range
• High-throughput kinetic experiments to determine k_on, k_off and K_d of interaction pairs
• 32 x 32 Epitope binning assay in 8 hours; Complete a 96 x 96 binning matrix in 5 days

Download Antibody Solution Application Note

Kinetic characterization of Anti-fluoreszine and fluoreszine (332 Da). The innovative three-dimensional surface chemistry of the new SMAP biosensors drastically increases the binding capacity and thereby enhances sensitivity when working with biotinylated molecules.

Long-term baseline stability is a key element when measuring slow dissociation rates. Gator Bios improved thermal insulation of the optical bench greatly reduces background noise which results in a clearer signal. This makes it possible to extend the measurement time in the dissociation step and to precisely measure high affinity interactions up to picomolar range. Accelerate your antibody discovery by identifying strong candidates early and with high accuracy.

Kinetic characterization of TNF-α and anti-TNF-α. A clean signal with little noise and minimal baseline drift enables extended off-rate measurements to determine picomolar interactions with high precision.

Gator Bio FLEX SA Kit

Kinetic characterization of TNF-α and anti-TNF-α for over 10 reactivations. The FLEX SA Kit lets you reactivate your Streptavidin sensors so you get more experiments done with less probes. The days of single use biosensors are over.

Regeneration profile: Concentration series of mouse IgG (10 µg/ml - 0.1 µg/ml) measured 20 times with sensor regeneration. The improved biosensor surface chemistry allows for more regeneration cycles without a loss in binding capacity.

• Wide dynamic range: The improved biosensor surface chemistry increases the concentration range for quantitations
• Unique applications: Quantify your AAV samples with the new ready-to-use AAVX-probes
• User friendly: The intuitive software enables fast and easy assay setup and analysis - even for users with little experience
• Customer support: Gator Bio listens to customer feedback and incorporates it into future releases and updates
• Future prove: The frequent releases of new and improved biosensors ensure that your assays are always performed with state-of-the-art technology

Biolayer Interferometry (BLI) is an established method that is traditionally used to study biomolecular interactions. This label-free technique measures the binding of biomolecules to the tip of a biosensor in real-time to determine kinetic rates of interaction pairs or to quantify biomolecular samples. The newest generation of AAV biosensors from Gator Bio opens up this application to the field of viral vectors and enables accurate and reliable quantitation of AAV total capsid titer. With the AAVX and AAV9 biosensors and the new high-sensitivity kits, Gator covers AAV serotypes 1 – 10 with a dynamic range from 5E+6 to 1E+13 vp/ml and a CV of <10%.

The new Empty/Full Ratio Kit is a practical and cost-effective solution for a challenging step in AAV development. With this new tool, the Empty/Full ratio of AAV samples can be determined after purification or in crude extracts with a dynamic range from 5% - 100% Full and 10% Resolution. A throughput of 8 samples in 40 minutes and a minimal sample volume of only 40 µl makes this kit not only a valuable asset in research, but also for upstream and downstream processes. Gator Bios AAV portfolio offers a single flexible technique with multiple applications in research and development of viral vectors that offer high accuracy, short assay times and a wide dynamic range.

• Robust: AAV titer and Empty/Full ratio can be determined in crude extracts, cell medium or purified samples.
• Low maintenance: Probes and sample plates are disposable. There is no need for extensive cleaning protocols or instrument maintenance.
• Cost effective: Automatic sensor regeneration keeps the assay cost low.
• Little material needed: Measurements can be performed with a minimal sample volume of only 40 µl.
• Low sample consumption: The Measurement is non-invasive. Precious sample material can be recovered after quantitation.
• User friendly: The intuitive software enables easy assay setup and analysis.

For more information, see the Application Note: Comparison of Gator® BLI and Progen ELISA for Quantitation of AAV Serotypes

Download AAV Quantitation Application Note