Opentrons® OT-2 pipetting robot – affordable and flexible lab automation

The Opentrons OT-2 benchtop pipetting robot is a flexible system designed to automate life science workflows that involve liquid handling. The instrument is based on a modular platform.  

Lab automation presents several challenges, and most solutions are either expensive and complex or easy to use but limited to specific workflows. The OT-2 robot fills this gap, allowing you to choose from a wide range of modules and pipettes.  

The Opentrons OT-2 robot will: 

  • Enable increased throughput 
  • Reduce hands-on time 
  • Improve consistency across samples and runs 
  • Help optimize time-consuming protocols 
  • Provide highly accurate results 

The list of applications for the Opentrons OT-2 liquid handler is endless. Commonly used applications are: 

  • Nucleic Acid Extraction 
  • PCR 
  • ELISA 
  • MGS Library preparation 
  • Reagent of sample aliquoting 
  • Serial dilutions 
  • Biochemical assays 
  • Small-scale protein purification  
  • LC-MS sample preparation 

In addition, the OT-2 is compatible with ANSI/SLAS-compliant labware and automation-friendly consumables, so you can keep using your own labware. 


The OT-2 is a flexible and easy-to-implement system for many different types of laboratories. Choose from the list of pre-designed workstations covering the most popular applications, or build your own system with the on-deck hardware modules and swappable 1- and 8-channel pipettes. 

Workstations include the OT-2 robot, modules, pipettes, and all necessary labware to accommodate your selected application. All workstations can be customized by adding or removing available hardware modules to suit your workflow. For more options and additional information, please get in touch with your local sales representative. 

Available workstations are: 

  • PCR workstation for PCR setup and thermocycling (max 96 samples) 
  • Cell assay-based workstation  
  • Pooling and normalization workstation  
  • Dual flow chromatography protein purification workstation (max 96 samples) 
  • Sample aliquoting workstation (max 96 samples) 


Available on-deck hardware modules: 

  • Temperature Module (GEN2) 
  • HEPA Module (GEN1) 
  • Magnetic Module (GEN2) 
  • Heater-Shaker Module 
  • Thermocycler Module 
  • Pipettes 

Software and protocols

The Opentrons app controls the robot and offers flexible options for creating individual protocols. The protocol software is adaptable to various workflows and does not require coding knowledge. Beginners can either create a protocol directly in the software or load established protocols from the library, while experienced programmers can use the open-source software Python API.

Opentrons OT-2 Teaser

Opentrons OT-2 versus the Opentrons Flex™ liquid handler

Are you in doubt about which robot will suit your needs best?  



  • Ideal for sample reformatting, reagent preparation, and routine workflows 
  • Fits in a biological safety cabinet 
  • Run sensitive contamination-prone applications by using the HEPA Filter module 


Opentrons Flex: 

  • Ideal for higher throughput and more complex workflows  
  • Maximizes walk-away time 
  • 1-, 8-, and 96-channel pipetting 
  • Integrated gripper – can move labware to and from slots on the deck 
  • Deck expander – gives you 4 additional deck slots for tips and labware 

Technical data

Dimensions (WxDxH) 63 × 57 × 66 cm
Weight 48 kg
Pipette overview Smart sensors support automatic calibration, real-time positioning, and error detection
Pipette volumes and specs

1-Channel Pipettes: 1–1000 µL

8-Channel Pipettes: 1–300 µL,


11 main ANSI/SLAS-format deck slots

Deck can be reconfigured by removing deck slots and replacing them with other deck features

Connectivity WiFi 2,4 GHz IEEE 802.11b/g/n, USB 2.0
Video Integrated video camera (included for future or custom functionality)
Audio Integrated audio system (Included for future or custom functionality)
Power requirements

Robot Power Input: 36 VDC, 6.1 A

Power Adapter Input: 100–240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC

Frame composition Rigid steel and CNC aluminum design
Operating temperature & relative humidity

Temperature: Recommended 20–25 °C

Humidity: Recommended 40–60% RH

1-channel accuracy & precision

15% accuracy at 1 μL

5% precision at 1 μL

Sterility Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment. Add the Opentrons HEPA Module, or add your own UV lights
API compatibility Opentrons software is open-source; integration of other software to interface is possible by the customer

Windows 10 or later, macOS 10.10 or later, Ubuntu 12.04 or later.

Opentrons App available

Tip compatibility

Use Opentrons Flex Tips and Opentrons Flex Filter Tips

Third party tip performance cannot be guaranteed by Opentrons.

Labware compatibility Use common labware already in our Labware Library, or add your own.
Warranty One year warranty against manufacturer defects
Certifications Certifications complete: CE, UL, FCC, ISO 9001




Technical Service

During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.

Detailed information about our support options can be found here.


Opentrons believes that scientists should be able to focus on their science to accelerate discovery and innovation, and that access to automation is a key tool in achieving this goal. Their mission is to democratize access to laboratory robotics, providing the scientific community with a common platform to easily share protocols and reproduce each other’s results. There are currently ten times the number of scientists to lab robots in the world. Opentrons envisions a future in which that ratio will be flipped, and there will be ten times the number of lab robots to scientists. Headquartered in New York, Opentrons operate globally.

More about Opentrons Labworks https://opentrons.com

125 employees
10 locations in europe
32 years of experience
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