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Protein Fluidics
Pu•MA System™ 3D
Automated 3D cell-based Assays
The Pu•MA System 3D has been designed to automate assays for spheroids or organoids by maintaining the 3D cell model in a special designed flowchip to allow automated media or reagent exchanges.
- Automated workflow for complex assay protocols
- Protected sample chamber to prevent cell damage
- Automatic execution of media change, compound additions and supernatant sampling
- Small well with low reagent volume
- Compatible with high content imaging and plate reader systems
- Small footprint fits on bench and incubator
- 3D Cell Culture
- Automation
- Sample Processing
- Sample Preparation
- Stem Cell Cultivation
- Cell Culture
- Cell Biology
- Belgium
- Denmark
- Germany
- Finland
- France
- Luxembourg
- Netherlands
- Norway
- Austria
- Sweden
- Switzerland
Pu•MA System™ 3D
The Protein Fluidics’ Pu·MA System 3D improves your workflow to automate assays with minimal user handling. The open platform allows you to program complex protocols and increases the reproducibility of your results. The 3D model is placed in a protected Chamber with microfluidic liquid exchanges that eliminates temperature and mechanical perturbations of 3D cell models. The Pu·MA System and Flowchips use valve-less fluidic switching (VLFS) to precisely control fluid movement in the flowchip. Preloaded protocols allow easy handling of the device and execute all fluid transfers and incubation steps. The thin clear bottom of the Flowchip is compatible with imaging and standard plate reader systems (384 wells). The system can fit inside any standard cell culture incubator and maintains the 3D cell models under physiological conditions while the assay steps are in progress, which reduces stress and damage to the cells.
Applications:
- Toxicology: Toxicity testing in spheroids with in situ imaging
- Metabolism: Pu·MA System 3D for single spheroids assays and downstream metabolomics
- Neuroscience: Phenotypic response to sequential drug addition and analysis in neurospheroids
- Oncology: Automated organoid assays, sequential drug additions, in situ sampling and in situ imaging
Automate your assay – not only for single organoids
Example for applications
Protein Fluidics | Pu•MA System 3D | Automated 3D cell-based Assays
Technical data
| Performance | 8 - 32 samples per run 1 - 5 days processing time 10 - 20 µl reagent volume |
| Operating Conditions | Ambient temperature: 20 - 40 °C Relative humidiy: 15-95 % (non condensing) |
| Software | Touchscreen driven interface Pu•MA Software |
| connections | 2x USB 2.0 Port (front) 1x RS-232 9 pin serial port (rear) |
| Electrical Requirements | Power requirements: 12 VDC input voltage 110 - 240 VAC at 50-60 Hz 3,3 A current 30 W |
| Clearance | 15,2 cm from all sites (6 in) |
| Physical Dimensions | Width: 305 mm (12 in) Depth: 355 mm (14 in) Height: 203 mm (8 in) Weight: 11,4 kg (25 lbs) |
Consumables
| 3D Cell Flowchips - Pack (4) | Protein Fluidics |
| 3D Cell Flowchips - Big Pack (12) | Protein Fluidics |
| Membrane | Protein Fluidics |
| Blanking Chips (4) | Protein Fluidics |
Technical Service
During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.
Detailed information about our support options can be found here.
The privately held company Protein Fluidics Inc. is located in the San Francisco Bay Area. The company is commercializing assay automation platforms using novel microfluidic technologies to accelerate data driven decisions in the Life Sciences. It provides scientists with the latest automation tools to help with the next breakthrough discovery in oncology, diagnostics, and more. The Pu•MA System 3D for your 3D cell models, organoids or spheroids, maintains the cells in our specially designed flowchips to allow for easy media, metabolite or reagent exchanges for your oncology, toxicity, or metabolomics research.
More about Protein Fluidics: https://proteinfluidics.com
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