Pu•MA System™ 3D – Assay automation for spheroids or organoids

The Pu•MA System 3D offers numerous advantages for cell cultivation and drug discovery. Firstly, it enables an automated workflow for complex protocols, ensuring high reproducibility of results. Secondly, the sample chamber protects cells from mechanical stress and guarantees gentle cell treatment. Additionally, the system performs automatic media changes, drug additions, and supernatant sampling. The small well format with a small sample volume allows for cost-efficient experimentation and is suitable for analyzing rare cells or limited samples. The system is also compatible with high-content imaging and plate readers and features a space-saving design for laboratory and incubator use. The intuitive touchscreen interface facilitates operation and enables quick familiarization with the system.

The Pu•MA System 3D is perfectly suited for a broad range of applications, including toxicology, metabolism, neuroscience, and oncology. The automated sequential drug addition and in situ imaging and sampling make the system particularly effective in oncology and neuroscience. In toxicology, the system can be used for toxicity testing in spheroids with in situ imaging. In metabolism, it can perform single-spheroid assays and subsequent metabolomics analyses.

With the Pu•MA System 3D, you can achieve precise control and automation of 3D cell assays, allowing you to program complex protocols and increase the reproducibility of results. The use of the Pu•MA System 3D is an effective and efficient way to automate your 3D assays and accelerate your research.



  •  Toxicology: Toxicity testing in spheroids with in situ imaging
  •  Metabolism: Pu•MA System 3D for single spheroids assays and downstream metabolomics
  •  Neuroscience: Phenotypic response to sequential drug addition and analysis in neurospheroids
  •  Oncology: Automated organoid assays, sequential drug additions, in situ sampling and in situ imaging



Automate your assay – not only for single organoids

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Example for applications

Protein Fluidics | Pu•MA System 3D | Automated 3D cell-based Assays

Technical data



Performance 8 - 32 samples per run
1 - 5 days processing time
10 - 20 µl reagent volume
Operating Conditions  Ambient temperature: 20 - 40 °C
Relative humidiy: 15-95 % (non condensing) 
Software Touchscreen driven interface 
Pu•MA Software
connections 2x USB 2.0 Port (front)
1x RS-232 9 pin serial port (rear)
Electrical Requirements   Power requirements: 12 VDC input voltage
110 - 240 VAC at 50-60 Hz
3,3 A current
30 W
Clearance 15,2 cm from all sites (6 in)
Physical Dimensions   Width: 305 mm (12 in)
Depth: 355 mm (14 in)
Height: 203 mm (8 in)
Weight: 11,4 kg (25 lbs)







PF-7000-0053 Membrane
PF-7000-0081 3D Cell Flowchips (4)
PF-7000-0082 3D Cell Flowchips (12)


Technical Service

During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.

Detailed information about our support options can be found here.

Logo Protein Fluidics
Protein Fluidics

The privately held company Protein Fluidics Inc. is located in the San Francisco Bay Area. The company is commercializing assay automation platforms using novel microfluidic technologies to accelerate data driven decisions in the Life Sciences. It provides scientists with the latest automation tools to help with the next breakthrough discovery in oncology, diagnostics, and more. The Pu•MA System 3D for your 3D cell models, organoids or spheroids, maintains the cells in our specially designed flowchips to allow for easy media, metabolite or reagent exchanges for your oncology, toxicity, or metabolomics research.

More about Protein Fluidics: https://proteinfluidics.com

125 employees
10 locations in europe
32 years of experience
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