![[Translate to EN:] Protein Fluidics Pu•MA System 3D](/fileadmin/_processed_/2/5/csm_IMG_0583_fc5a678456.png)
Protein Fluidics
Pu•MA System™ EC
Automated 3D cell-based Assays
The Pu•MA System EC automates assays for spheroids/organoids under controlled conditions by placing the 3D model in a specially designed microfluidic flowchip. The instrument controls the required culture conditions (temperature and CO2 content) and performs individually adjustable reagent or media transfers in a fully automated manner. This enables automated assays for drug testing, co-cultures and IF staining.
- Automated workflow for complex assay protocols
- Protected sample chamber to prevent cell damage
- Automatic execution of media change, compound additions and supernatant sampling
- Compatible with high content imaging and plate reader systems
- Integrated temperature and CO2 control
- Small well with low reagent volume
- Automation
- Sample Processing
- Sample Preparation
- Stem Cell Cultivation
- Cell Culture
- Cell Biology
- Cell Therapy
- Belgium
- Denmark
- Germany
- Finland
- France
- Luxembourg
- Netherlands
- Norway
- Austria
- Sweden
- Switzerland
Pu•MA System™ EC – Automation of assays for spheroids or organoids
The Protein Fluidics’ Pu•MA System EC improves your workflow to automate assays with minimal user handling. The open platform allows you to program complex protocols and increases the reproducibility of your results. The 3D model is placed in a protected chamber with microfluidic liquid exchanges that eliminates mechanical perturbations of 3D cell models. The Pu•MA System EC and Flowchips use valve-less fluidic switching (VLFS) to precisely control fluid movement in the flowchip. Customizable protocols allow easy handling of the device and execute all fluid transfers and incubation steps. The thin clear bottom of the Flowchip is compatible with imaging and standard plate reader systems (384 wells).
The system with it interne temperature and CO2 control maintains the 3D cell models under physiological conditions while the assay steps are in progress, which reduces stress and damage to the cells. for longer cultivation periods, the reservoir flowchip holder allows a humidity of >80% to prevent cell from drying out.
Applications
- Toxicology: Toxicity testing in spheroids with in situ imaging
- Metabolism: Pu•MA System 3D for single spheroids assays and downstream metabolomics
- Neuroscience: Phenotypic response to sequential drug addition and analysis in neurospheroids
- Oncology: Automated organoid assays, sequential drug additions, in situ sampling and in situ imaging
Automate your assay – not only for single organoids
Technical data
| Performance | 8 - 32 samples per run 1 - 5 days processing time 10 - 20 µl reagent volume |
| Operating Conditions | Ambient temperature: 20 - 40 °C Relative humidiy: 15-95% (non condensing) |
| Software | Touchscreen driven interface Pu•MA Software |
| Connections | 2x USB 2.0 Port (front) 1x RS-232 9 pin serial port (rear) |
| Electrical Requirements | Power requirements: 12 VDC input voltage 110 - 240 VAC at 50-60 Hz 3,3 A current 30 W |
| Environmental control | CO2: 1% -10% Temperature: +5 °C above ambient to 40 °C >80% humidity with reservoir |
| Physical Dimensions | Width: 305 mm (12 in) Depth: 355 mm (14 in) Height: 203 mm (8 in) |
Consumables
| 3D Cell Flowchips - Pack (4) | Protein Fluidics |
| 3D Cell Flowchips - Big Pack (12) | Protein Fluidics |
| Membrane | Protein Fluidics |
| Blanking Chips (4) | Protein Fluidics |
| Flow Chip holder | Protein Fluidics |
| reservoir flow chip holder | Protein Fluidics |
| imaging flow chip holder | Protein Fluidics |
| 3D mag loading tray | Protein Fluidics |
Technical Service
During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.
Detailed information about our support options can be found here.
The privately held company Protein Fluidics Inc. is located in the San Francisco Bay Area. The company is commercializing assay automation platforms using novel microfluidic technologies to accelerate data driven decisions in the Life Sciences. It provides scientists with the latest automation tools to help with the next breakthrough discovery in oncology, diagnostics, and more. The Pu•MA System 3D for your 3D cell models, organoids or spheroids, maintains the cells in our specially designed flowchips to allow for easy media, metabolite or reagent exchanges for your oncology, toxicity, or metabolomics research.
More about Protein Fluidics: https://proteinfluidics.com
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