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BioFlux™ One Live Cell Imaging System

The BioFlux™ One system is the simple solution for labs that want to run shear flow cell experiments on their current microscope, but don't need 2-phase flow or high throughput. It offers all the benefits of the industry standard BioFlux system, but at a price point similar to a "home brew" flow cell setup. The BioFlux system is the established standard for a wide range of cellular interaction assays under physiological shear flow conditions. The BioFlux One system provides the ability to perform complex modelling of the in vivo cell environment, in a package that every lab can afford.

The heart of the BioFlux One system is identical to the BioFlux 200 and the BioFlux 1000z systems: the computer-controlled pneumatic pumping system for automated precise control of flow cell experiments using Fluxion's unique microfluidic flow cell well plates. The BioFlux One can run up to 24 flow cell experiments simultaneously and is ideal for platelet aggregation, leukocyte adhesion, and biofilm analysis.

It works with an inverted microscope and is compatible with fluorescence, brightfield, phase, and confocal imaging. User-friendly software automates experimental protocols and provides a powerful analysis package. Upgrade capabilities allowing for 2-phase flow and/or higher throughput make the BioFlux One the perfect choice for functional assay research.

Technical data

 

BioFlux Controller

Shear flow range 0,1-200 dyne/cm2
Flow control  Single-phase (multi-stage flow requires BioFlux 200 or 1000z)
Dimension (W x L x H) 30 x 33 x 22 cm
Example Applications Biofilm formation, host/pathogen interactions, cell adhesion, leukocyte rolling, platelet aggregation, stem cell differentiation
System upgrades Heating plate/ temperature control, alternative gas supply (e.g. for hypoxia), pulsatile flow

 

 

BioFlux Microtitre plates

Plate format 48-well SBS standard plates, pre-sterilised. Each plate contains 24 experimental flow cells/channels
Plate construction The plates are available with these materials: 
• Channels made of PDMS and 170 μm coverslip glass bottom
• All PDMS construction
• Channels made of PDMS and glass bottom with user-definable surface (user attaches desired material using supplied adhesive layer)
Throughput  up to 24 simultaneous experiments
Dimension (W x H) of the microfluidic observation channel 350 x 70 µm

 

 

BioFlux Software

Operating modules Manual run, AuroRun Editor, Autorun, Image acquisition, Image analysis
Operating system requirements Windows 10 (or higher)
Memory 4 GB RAM
Available hard drive space 2 GB
Cables USB 2.0 connection

 

Consumables

BioFlux Plates

FL-910-0110-5Pack BioFlux 6-well plate 0-20 dyn
FL-910-0048-5Pack BioFlux 48-well plates, high shear, 0-200 dyn
FL-910-0047-5Pack BioFlux 48-well plates, low shear, 0-20 dyn
FL-910-0120-5Pack BioFlux 48-well custom substrate plate
FL-910-0051-5Pack BioFlux 24-well plate 0-20 dyn
FL-910-0121-5Pack BioFlux 48-well 0-20 dyn PDMS bottom plates
FL-910-0145-5Pack BioFlux 24-well 0-20 dyn PDMS bottom plates

 

System & Upgrades

FL-950-0146 BioFlux One mit 48-Well-Druckverteiler und Dampfsperre - ohne Temperaturkontrolle. Inklusive Software und Zubehörsatz.
FL-950-0106 Temperature control (heater) upgrade for BioFlux One
FL-950-0035 Environmental control. Enables assays under hypoxia or alternative gas. Enables use of premixed CO2 or other gas mixture.
FL-940-0020 BioFlux Pulsatile Flow Module. Provides capability to mimic
FL-950-0038 Quattro Multiplate adapter for 48-well plates (no heaters). Enables use of up to 4 BioFlux plates in parallel and includes 3 BioFlux 48- well plate interfaces with quick-release fastener.

 

Accessories

FL-800-0069 Gasket for 48-well interface.
FL-910 0017 Qty 16 0.2 uM replacement filters for BioFlux controller

 

Technical Service


During our business hours, our free telephone support will be glad to assist you in case of any questions. Our Technicians take care of the delivery and installation of original spare parts of the highest quality as well as certified testing, measuring, and control equipment. Our maintenance according to manufacturer specifications includes functional tests, cleaning, accuracy testing with calibration as well as a detailed report. In addition, our maintenance contracts protect your investments and reduce the total cost of ownership.

Detailed information about our support options can be found here.

Logo Fluxion Biosciences
Fluxion Biosciences

Fluxion Biosciences is located in California and delivers advanced solutions that automate complex cell-based and cell-free assays. Products include ERASE-Seq for NGS analysis of cancer from a "liquid biopsy"blood sample, IsoFlux for circulating tumor cell analysis, BioFlux for functional analysis of cellular interactions, and the IonFlux automated patch clamp system. Hundreds of labs worldwide rely on Fluxion's solutions to research novel biology, accelerate drug discovery, and tailor therapies based on patient-specific biomarkers.

More about Fluxion Biosciences: www.fluxionbio.com

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125 employees
10 locations in europe
32 years of experience